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Your position:  Home >> Sales promotion >> 
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ExoSAP-IT Reagent Promotion——30% off
2012-6-7

ExoSAP-IT Reagent Promotion——30 off

ExoSAP-IT reagent has been referenced in thousands of publications since 2000; the market leader in enzymatic PCR clean-up with an established reputation for consistency and quality.

Purchase it and enjoy its high efficiency and convenience.

Recover 100% of your PCR product with USB ExoSAP-IT PCR Product Clean-Up

Achieve high data quality from PCR products

ExoSAP-IT reagent may be used as an effective clean-up method prior to fluorescent or radioactive DNA sequencing, SNP analysis, or any other application requiring a PCR product free of excess nucleotides and primers.

The data below shows the results of downstream processing with PCR product cleaned up by either ExoSAP-IT reagent, columns, or ExoStar product. The ExoSAP-IT reagent data shows no miscalls or frame shift mutations.

 

Fig. 1. Fluorescent sequencing results of a 1,007 bp PCR product. A 1,007 bp fragment was amplified and treated with ExoSAP-IT reagent (above) or ExoStar product (below) and sequenced. Pherograms revealed no miscalls with ExoSAP-IT reagent but four miscalls at position 25, 26, 30, and 39 with ExoStar product. 5 µl of PCR product was treated with 2 µl ExoSAP-IT reagent or 1 μl illustra Exo I and 1 µl illustra alkaline phosphatase from the ExoStar 2-tube formulation. Note: Two of the four miscalls are frame-shifts.
Fig. 2. Fluorescent sequencing results of a 100 bp pUC18 PCR fragment sequenced with a -20 Fwd primer using fluorescent sequencing reagents. PCR clean-up performed with: (a) ExoSAP-IT reagent;(b) a column designed for PCR clean-up. Base miscalls in (b) are due to inherently low yields of short PCR products when using columns.

Recover 100% of your PCR product with USB ExoSAP-IT PCR Product Clean-Up:
the gold standard for enzymatic PCR clean-up.

·                     100% sample recovery with one-tube, one-step PCR clean-up — no loss of PCR products, regardless of the fragment size

·                     Expertise from the original and only source of ExoSAP-IT reagent — the USB team has been producing, testing, and selling ExoSAP-IT reagent since 2000

·                     The gold standard in enzymatic PCR clean-up — ExoSAP-IT reagent has been referenced in thousands of publications since 2000; the market leader in enzymatic PCR clean-up with an established reputation for consistency and quality

·                     Quality results with SAP — only ExoSAP-IT reagent uses SAP, offering higher specific activity for better sequencing data when compared to alternative alkaline phosphatases

·                     Optimized formulation — the buffer formulation in ExoSAP-IT reagent has been optimized to give superior performance of the product

·                     Eliminate spin columns — decrease time and expense while increasing yield

·                     Degrades contaminating primers and dNTPs — no interference in downstream applications

·                     Green option — generate less waste with our single-tube solution to PCR purification vs. columns

·                     Sole source — Affymetrix is the only supplier of USB ExoSAP-IT reagent, ensuring your lab of product consistency and integrity

·                     Why lose time validating an alternate source — only ExoSAP-IT reagent has been around long enough to have validated stability measurements

ExoSAP-IT reagent is designed for simple, quick PCR clean-up for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR product mixture will interfere with these methods. ExoSAP-IT reagent removes these contaminants.

How it works

ExoSAP-IT reagent is added directly to the PCR product and incubated at 37°C for 15 minutes. After PCR treatment,
   ExoSAP-IT reagent is inactivated simply by heating to 80°C for 15 minutes.

ExoSAP-IT single-step PCR clean-up utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase, together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. Exo I removes residual single-stranded primers and any extraneous single-stranded DNA produced in the PCR. SAP removes the remaining dNTPs from the PCR mixture.

Simple: single-step

This method is designed to require a minimum of ‘hands-on’ time. Enzymatic removal of excess primers and unincorporated nucleotides occurs in one easy step by using ExoSAP-IT reagent in a single tube or microtiter well.

No sample loss

Use of the ExoSAP-IT method eliminates all gel or column purifications, sedimentations, filtrations, beads and/or magnetic separations. There is 100% recovery of both short and long PCR products with ExoSAP-IT reagent.

Use the ExoSAP-IT method for PCR Clean-Up and see the difference 100% can make.

Item

Product Name

Unit Size

Price

78250 40 UL

ExoSAP-IT For PCR Product Clean-Up

20 reactions

273.00

78200 200 UL

ExoSAP-IT For PCR Product Clean-Up

100 reactions

756.00

78201 1 ML

ExoSAP-IT For PCR Product Clean-Up

500 reactions

2,961.00

78202 4X1 ML

ExoSAP-IT For PCR Product Clean-Up

2000 reactions

10,633.00

78205 10 ML

ExoSAP-IT For PCR Product Clean-Up

5000 reactions

24,150.00

Exonuclease I

Item

Product Name

Unit Size

Price

70073Z 2500 UN

Exonuclease I, 10 units/µl

2500units

574.00

70073X 5000 UN

Exonuclease I, 10 units/µl

5000units

1064.00

72073 5 KU

Exonuclease I, 20 units/µl

5000units

1099.00

SAP

Item

Product Name

Unit Size

Price

78390 500 UN

Shrimp Alkaline Phosphatase (SAP)

500units

560.00

78390 1000 UN

Shrimp Alkaline Phosphatase (SAP)

1000units

959.00

78390 5000 UN

Shrimp Alkaline Phosphatase (SAP)

5000units

3297.00

 

 
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  • 游客『Lesley』于2016-10-15 6:12:34发表评论:
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